The leucine zipper of TFE3 dictates helix-loop-helix dimerization specificity.
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منابع مشابه
The leucine zipper of TFE3 dictates helix-loop-helix dimerization specificity.
TFE3 is a DNA-binding protein that activates transcription through the muE3 site of the immunoglobulin heavy-chain enhancer. Its amino acid sequence reveals two putative protein dimerization motifs: a helix-loop-helix (HLH) and an adjacent leucine zipper. We show here that both of these motifs are necessary for TFE3 to homodimerize and to bind DNA in vitro. Using a dominant negative TFE3 mutant...
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The plant hormone auxin can be regulated by formation and hydrolysis of amide-linked indole-3-acetic acid (IAA) conjugates. Here, we report the characterization of the dominant Arabidopsis iaa-leucine resistant3 (ilr3-1) mutant, which has reduced sensitivity to IAA-Leu and IAA-Phe, while retaining wild-type responses to free IAA. The gene defective in ilr3-1 encodes a basic helix-loop-helix leu...
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The murine homologue of the TFEC was cloned as part of an analysis of the expression of the microphthalmia-TFE (MiT) subfamily of transcription factors in macrophages. TFEC, which most likely acts as a transcriptional repressor in heterodimers with other MiT family members, was identified in cells of the mononuclear phagocyte lineage, coexpressed with all other known MiT subfamily members (Mitf...
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The MITF protein is a member of the MYC family of basic helix-loop-helix leucine zipper (bHLH-Zip) transcription factors and is most closely related to the TFE3, TFEC, and TFEB proteins. In the mouse, MITF is required for the development of several different cell types, including the retinal pigment epithelial (RPE) cells of the eye. In Mitf mutant mice, the presumptive RPE cells hyperprolifera...
متن کاملHelix-turn-helix, zinc-finger, and leucine-zipper motifs for eukaryotic transcriptional regulatory proteins.
Four distinct structural motifs have been proposed for the DNA-binding domains of eukaryotic transcriptional regulatory proteins; the helix-turn-helix, two kinds of zinc finger, and the leucine zipper. Within each structural motif, there are often families of related proteins that recognize similar DNA sequences and are conserved throughout the eukaryotic kingdom. However, the processes of tran...
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ژورنال
عنوان ژورنال: Genes & Development
سال: 1991
ISSN: 0890-9369
DOI: 10.1101/gad.5.6.1057